Generally - no
On the north west side of Greater Manchester - evidently yes.
This is of course partly a historic hangover - professional football developed in the industrial areas of Lancashire and the Midlands originally, with 6 Lancashire teams and 6 Midland teams comprising the original football league. It didn't develop in the south until much later and there were still only a handful of southern clubs in the FL by 1914. After WWI the Southern League was essentially incorporated as a Third Division. A year later it was decided to even this up by rebranding it as Third Division South and adding a Third Division North. D3N was always somewhat weaker than D3S. After creating D3 and D4 in 1958 (L1 and L2 in modern parlance) the majority of the clubs regularly seeking re-election were northern. After relegation to the Conference was introduced it seems to have had a sink or swim effect - some clubs such as Rochdale, Crewe and Scunthorpe have improved markedly from once being bottom feeders, others have sunk without trace.
A bit of a diversion from the question, that. In answer to the question, I would always say no simply because most professional clubs sustain crowds (or did in the BCV era) which should be sufficient if well run. Supporters Trust run clubs such as (my club) Exeter, Wimbledon and Wycombe are testament to that. English clubs get crowds and media attention at lower levels which are simply unprecedented anywhere else. Exeter average around 4000 and that isn't anything remarkable for L2. This is in part due to the population density of England and relative compactness of the dense populations. There is of course not much at the fringes - no teams in Cornwall, one in Kent, nothing north of Newcastle, two teams in Cumbria (welcome back Barrow), one in the Marches and two in Norfolk and Suffolk. But then there aren't many people there.
In the other big 5 European leagues, Germany seems to have a similar level of interest in lower league football, but its greater size prevents more national divisions (it only acquired a Bundesliga III relatively recently). Buli III however seems similar to England - some teams with 5 figure crowds, others closer to 2000. Spain and Italy seem to have little interest in level 2 football never mind lower - the Spanish Segunda Division B 9L1 equivalent) has numerous teams with sub-1000 average attendances. Save for a few pockets such as Lens, St Etienne and Marseille, France barely seems to have any interest in Ligue Un, never mind anything lower than that.
English football has survived with 92 professional league clubs and numerous non-league ones for many years. As long as they're prudently run it isn't a problem. As long as people are allowed into the ground. That last point might be problematic.